By Sukriti Nag
Univ. of Toronto, Canada. textual content presents the main points of chosen morphologic, permeability, shipping, in vitro, and molecular suggestions for BBB experiences. each one half is preceded by way of a assessment emphasizing the benefits and pitfalls of specific innovations. Written for researchers in BBB. DNLM: Blood--Brain Barrier--physiology.
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Pardo, C. , and Trapp, B. D. (1994) Detection of MHC class II-antigens on macrophages and microglia, but not on astrocytes and endothelia in active multiple sclerosis lesions. J. Neuroimmunol. 51, 135–146. 296. , and Mork, S. (1994) HLA class II molecules (HLA-DR, DP, DQ) on cells in the human CNS studied in situ and in vitro. Immunology 82, 535–541. 297. , et al. (1995) The costimulatory molecule B7 is expressed on human microglia in culture and in multiple sclerosis acute lesions. J. Neuropathol.
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7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 49 different labeled containers, which are disposed by the biohazard department of the institution. Distilled water filtered using a no. 50 Whatman filter is used for the preparation of stains for ultrathin sections and to fill the trough adjacent to knives. Undiluted fixative can be frozen in 250-mL amounts in Nalgene flasks. 1 M cacodylate buffer containing sucrose for perfusion. Some researchers perfuse a buffer or Ringer’s solution containing heparin for few minutes to clear the blood followed by the perfusion of the fixative solution.
