By John R. Masters (Editor), Bernhard O. Palsson (Editor), James A. Thomson (Editor)
If you want to develop or signify embryonic stem cells or convince them to distinguish right into a specific cellphone sort, then this e-book includes details that's very important for your good fortune. the purpose is to supply transparent basic directions and protocols for starting to be, retaining and characterizing embryonic stem cells and information of a few of the equipment used to make stem cells differentiate into particular phone forms. The contents can be of curiosity to stem mobilephone biologists, tissue engineers and scientists wishing to exploit embryonic stem cells for healing reasons. every one bankruptcy has been written and edited by way of the world over revered scientists operating on the innovative of technological advancements in human embryonic stem cells.
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Extra info for Embryonic Stem Cells (Human Cell Culture)
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23: 1534–1541. W. (2004) Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. , 22: 53–54. , et al. (2005) Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells. Hum. Mol. , 14: 3129–3140. , et al. (2006) CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells. Nat. , 24: 351–357. , and Lako, M. (2005) Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages.
Qxd 18/4/07 4:45 PM Page 41 CHAPTER 4 GENETIC MODIFICATION OF HUMAN EMBRYONIC STEM CELLS THOMAS P. ZWAKA Baylor College of Medicine, Center for Cell and Gene Therapy and Department of Molecular and Cellular Biology, Houston, Texas, USA 1. INTRODUCTION Embryonic stem (ES) cells are derived from an early embryonic stage during which the cellular machinery is geared toward rapid expansion and diversification, simultaneously possess both the ability for unlimited self-renewal and the potential to differentiate into derivatives of all three germ layers (Pedersen 1986).
And Minger, S. (2003) Preimplantation genetic diagnosis as a novel source of embryos for stem cell research. Reprod. Biomed. Online, 7: 353–364. R. (2005) Generation of a human embryonic stem cell line encoding the cystic fibrosis mutation deltaF508, using preimplantation genetic diagnosis. Reprod. Biomed. Online, 10: 390–397. , and Bongso, A. (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro [In Process Citation]. Nat. , 18: 399–404. , and Schatten, G.