By R. Verpoorte (auth.), Dr. Robert Verpoorte, Prof. Dr. A. W. Alfermann (eds.)
Plant secondary metabolism is an economically very important resource of good chemical substances, resembling medicinal drugs, pesticides, dyes, flavours, and fragrances. furthermore, vital features of crops similar to style, flavour, scent, color, or resistance opposed to pests and ailments also are relating to secondary metabolites.
The genetic amendment of crops is possible these days. What does the potential of engineering plant secondary metabolite pathways suggest? during this publication, to start with a common creation is given on plant secondary metabolism, via an summary of the prospective methods which may be used to change secondary metabolite pathways. In a sequence of chapters from quite a few specialists within the box, an outline is given of the cutting-edge for vital teams of secondary metabolites.
No books were released in this subject thus far. This booklet will hence be a different resource of knowledge for all these concerned with vegetation as chemical factories of excellent chemical substances and people concerned with the standard of nutrition and decorative vegetation. it will likely be important in educating graduate classes within the box of metabolic engineering in plants.
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Plant Mol Bioi 1992;18: 1121-1131. 109 Stevens LH, BJorn TJM and Verpoorte R. Subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in cell suspension cultures of Catharanthus roseus and Tabernaemontana divaricata. Plant Cell Rep1993 ;12:573-576. 110 Stevens LH. Formation and conversion of strictosidine in the biosynthesis of monoterpenoid indole and quinoline alkaloids. PhD Thesis, Leiden, 1994. 111 DeLuca V and Cutler AJ. Subcellular localization of enzymes involved in indole alkaloid biosynthesis in Catharanthus roseus.
Anthranilate synthase, the first enzyme in the tryptophan branch is strongly inhibited by its end product, tryptophan. In the branch leading to phenylalanine, the first enzyme chorismate mutase is induced by tryptophan and inhibited by phenylalanine and tyrosine. 34·36 Even pH might be a regulatory factor, as was described for the hydroxylase converting coumaryl-CoA into caffeoyl-CoA in parsley. 5). Only after elicitation, when the intracellular pH drops, the enzyme becomes active. 104 Compartmentation Also compartmentation is an important aspect of regulation, both at cellular and subcellular level.
The example of the indole alkaloid biosynthesis clearly shows that selective transport proteins and/or pH-controlling genes may play an important role in the regulation of secondary metabolism. On top of subcellular compartmentation, even molecular compartmentation may occur. g. 57 -123 -124 -125)_ For example, separate metabolic channels are thought to occur in the endoplasmic Secondary metabolism 23 reticulum for the biosynthesis of sesquiterpenes and sterols. 123 •125 Also the pathway from phenylalanine to flavonoids is thought to occur in the endoplasmic reticulum involving a multienzyme complex, with the first (PAL) and the last step (a flavonoid glucosyltransferase) located in the lumen of the membranes.