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Extra info for Paint & Coatings Industry 2010,06
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In an attempt to create a cavity in the central core, arginines were incorporated at positions 11, 15, 30 and 33 in the second JR2E sequence. To avoid excessive charge repulsion at the interface between the four-helix bundle subunits the lysines in positions 7, 14 and 29 of the second JR2E sequence were replaced by alanines. The sequence of JR94K is related to that of JR2K in the same way that JR94E is related to that of JR2E with the difference that the interface residues and accompanying modifications were located in the first JR2K sequence (Figure 11).
In this pH range JR2EC and JR2ECref were negatively charged and repulsive forces prevented the particles from aggregating. At a pH below 5 the charged glutamic acids are protonated and a distinct red shift was observed for both JR2EC- and JR2ECref-functionalised gold particles due to aggregation (Figure 27). However, for JR2ECref-functionalised nanoparticles a much larger red shift was observed indicating a smaller interparticle distance[64]. Since JR2ECref is unable to fold it was expected to collapse in an unordered conformation on the surface of the particle and allow the nanoparticles to come close to one another once JR2ECref was neutralised.
P1, P2 = acid-labile side-chain protecting groups. 3. Matrix assisted laser desorption ionisation time of flight (MALDI-TOF) MALDI-TOF is a powerful and easy method for determining the molecular weight of large non-volatile molecules such as polypeptides and proteins[66, 67]. The analyte is co-crystallised with a vast excess of a matrix compound, in this work α-cyano-4hydroxycinnamic acid, on a metal plate and introduced into a high vacuum chamber. The sample is then irradiated with nanosecond laser pulses causing the sample to 46 evaporate.