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2007) and for rpoA-RNA polymerase alpha subunit gene by Naser et al. (2007). The main advantage of the application of genes that usually appear in one copy is that they enable accurate quantification by real-time PCR also in the mixed populations of bacteria belonging to different species, while the number of rRNA genes copies differs among the species. 2 Real-time PCR quantification of probiotic bacteria in non-dairy products It is well known that many food ingredients, including fats, proteins, divalent cations, and phenolic compounds, can act as PCR inhibitors.
2011) P. freudenreichii and Lb. , 2010) Application of PCR-Based Methods to Dairy Products and to Non-Dairy Probiotic Products Target population Target sequence Food matrix References Lb. helveticus and Str. , 2012) Str. , 2012) Lb. casei, P. pentosaceus, Str. , Pseudomonas spp. , 2003) S. , 2010) S. , 2009) Table 2. Examples of applications of reverse-transcription qPCR to dairy products. 29 30 Polymerase Chain Reaction 3. 1 Nucleic acid extraction from non-dairy probiotic products Probiotic products comprise probiotic dairy products and probiotic food supplements which appear in several forms, like powders, capsules, tablets, suspensions etc.
Treven et al. (submitted) evaluated the possibility of using bacteriocin-specific primers for the detection and quantification of Lb. , 2010). Two pairs of primers, namely GasA_401/610F/R and GasB_26102807F/R showed specificity for total gene cluster of gassericin K7 A (Genbank EF392861) or gassericin K7 B (Genbank AY307382) respectively as established by PCR assays using DNA of 18 reference strains belonging to Lb. acidophilus group and 45 faecal samples of adult volunteers who have never consumed K7 strain.
