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Sex pilus specific phages and procedures for their use are described in Chapter 3. Plasmids may also interfere with phage propagation. , 1981). , 1976), or more general effects on eel1 functions (Britton and Haselkom, 1975; Condit, 1976). In P. aeruginosa P A 0 and E. , 1977; Jacoby, 1986). Acknowledgements I gratefully acknowledge the use of material dealing with plasmid curing taken from the contribution of Car0 et al. from the 1st edition of this book. I am grateful to S. T. Fong for assistancewith the manuscript and preparation of the typescript.
Cofi and P . aeruginosa (Bacquero et a f . , 1978), methyl- 34 V. A. STANISICH enomycin A from Strep. , 1986) and possibly nisin from Strep. , 1975). Production of these types of substances can be demonstrated by exposure of a sensitive bacterium to the producer strain or culture supernatants of the producer strain. For example, the producer strain can be grown as single colonies or as a streak on nutrient medium and then overlayed with agar containing the sensitive bacterium. Alternatively, bacteria-free culture supernatants of the producer strain can be spotted on lawns of the sensitive bacteria.
1981). In “Molecular Biology, Pathogenicity and Ecology of Bacterial Plasmids” (S. Levy, R. C. Clowes and E. L. Koenig, Eds), pp. 217-226. Plenum Press, New York. Bradley, D. E. (1983). J. Gen. Microbiol. 29, 2545-2556. Bradley, D. , Taylor, D. E. and Cohen, D. R. (1980). J. Bacteriol. 143, 1466-1470. Bramucci, M. G. and Lovett, P. S. (1976). J . Bacteriol. 127, 829-831. , Hinkkanen, A. E. and Decker, K. (1982). Arch. Microbiol. 132,26-30. Brewin, N. , Wood, E. A. and Young, J. P. W. (1983). J.
